Knockout of circRNAs by base editing back-splice sites of circularized exons

Knockout of circRNAs by base editing back-splice sites of circularized exons

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Abstract Many circular RNAs (circRNAs) are produced from back-splicing of exons of precursor mRNAs and are generally co-expressed with cognate linear RNAs.Methods for circRNA-specific knockout are lacking, largely due to sequence overlaps between forms.Here, we use base editors cmpk bolus (BEs) for circRNA depletion.By targeting splice sites involved in both back-splicing and canonical splicing, BEs can repress circular and linear RNAs.Targeting sites predominantly for circRNA biogenesis, BEs could efficiently repress the production of circular but not linear RNAs.

As hundreds of exons are predominantly sgt grit discount coupon back-spliced to produce circRNAs, this provides an efficient method to deplete circRNAs for functional study.